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目的分析间接红细胞凝集试验(IHA)和金标免疫法(DIGFA)诊断血吸虫病的可靠性。方法采集经粪便孵化法确诊且未经治疗的血吸虫病患者血清标本46份和健康人血清42份,分别用IHA和DIGFA进行检测,观察2种方法的敏感度和特异度。IHA法每份血清做1∶5、1∶10、1∶20、1∶40共4个滴度。结果46份血吸虫病患者血清检测结果:IHA法1∶5、1∶10、1∶20、1∶40滴度检出率分别为91.30%(42/46)、76.09%(35/46)、65.22%(30/46)、45.65%(21/46),DIGFA法检出率为69.57%(32/46);42份健康人血清检测结果:IHA法1∶5、1∶10、1∶20、1∶40滴度阳性率分别为7.14%(3/42)、4.76%(2/42)、0%(0/42)、0%(0/42),IHA(1∶5)和IHA(1∶10)特异度分别为92.85%、95.24%,DIGFA的特异度为97.62%(41/42)。结论IHA和DIGFA检测血吸虫病患者血清阳性率(敏感度)偏低;用IHA和DIGFA诊断血吸虫病可信度较差;IHA的阳性阈值滴度为1∶5阳性检出率较高。
Objective To analyze the reliability of diagnosis of schistosomiasis by indirect hemagglutination test (IHA) and gold standard immunization (DIGFA). Methods 46 serum samples of healthy schistosomiasis patients diagnosed by excrement hatching method and 42 serum samples from healthy volunteers were collected and detected by IHA and DIGFA respectively. The sensitivity and specificity of the two methods were observed. IHA method for each serum to do 1: 5, 1:10, 1:20, 1:40 a total of four titers. Results The results of sera from 46 schistosomiasis patients showed that the titer of IHA method was 91.30% (42/46), 76.09% (35/46), respectively, 65.22% (30/46) and 45.65% (21/46) respectively. The detection rate of DIGFA method was 69.57% (32/46) The positive rates of titers of 20 and 1:40 were 7.14% (3/42), 4.76% (2/42), 0% (0/42), 0% (0/42), IHA The specificity of IHA (1:10) was 92.85% and 95.24%, respectively. The specificity of DIGFA was 97.62% (41/42). Conclusions The positive rate (sensitivity) of IHA and DIGFA in detecting schistosomiasis patients is low. The reliability of using IHA and DIGFA to diagnose schistosomiasis is poor. The positive detection rate of IHA with a positive threshold titer of 1: 5 is higher.