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目的 :研究载脂蛋白 E(Apo E)基因多态性与 2型糖尿病患者合并冠心病的关系。方法 :用聚合酶链反应 -限制性片段长度多态性 (PCR- RFL P) ,研究了 12 5例 2型糖尿病 (DM)患者和 71例健康体检者 Apo E基因型 ,分析了 Apo E基因多态性与 2型糖尿病及其合并冠心病的关系。结果 :(1)糖尿病组 (DM)、糖尿病合并冠心病组 (DC)及对照组等位基因频率分别为 5 .5 %、89.7%、4.8% ,4.1%、85 .3%、10 .6 %和 5 .6 %、86 .6 %、7.8%。相互间 P均 >0 .0 5 ,说明 Apo E基因型与中国人的 2型糖尿病及合并冠心病的发生发展无相关性。 (2 )通过对不同 Apo E基因型的血胆固醇 (TC)、甘油三酯 (TG)、高密度脂蛋白 (HDL )及低密度脂蛋白 (L DL )水平比较 ,发现携ε4等位基因的个体血 TC高于携其他 ApoE等位基因的个体 ,而携ε2等位基因的个体血 TG的水平高于携ε3、ε4等位基因的个体 (P<0 .0 5 )。 (3)Apo E基因多态性与高血压 (HT)无显著相关性。 (4 )携ε2、ε3、ε4等位基因的个体之间冠心病的发生率差异无显著性。结论 :Apo E等位基因多态性不是 2型糖尿病合并冠心病的独立危险因素
AIM: To investigate the association between apolipoprotein E (Apo E) gene polymorphism and type 2 diabetes with coronary heart disease. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to study the Apo E genotypes in 125 patients with type 2 diabetes mellitus (DM) and 71 healthy individuals. The Apo E gene Relationship between Polymorphism and Type 2 Diabetes Mellitus and Coronary Heart Disease. Results: (1) The frequencies of alleles in DM group, DM group and control group were5.5%, 89.7%, 4.8%, 4.1%, 85.3% and10.6 % And 5.6%, 86.6% and 7.8% respectively. P> 0.05, indicating that the Apo E genotype had no correlation with the occurrence and development of type 2 diabetes mellitus and coronary heart disease in Chinese. (2) Comparing the levels of TC, TG, HDL and LDL of different Apo E genotypes, Individual TCs were higher in individuals with other ApoE alleles than in those with other ApoE alleles, whereas those with ε2 alleles had higher TG levels than those with ε3 and ε4 alleles (P <0.05). (3) There was no significant correlation between Apo E gene polymorphism and hypertension (HT). (4) There was no significant difference in the incidence of coronary heart disease between individuals with ε2, ε3 and ε4 alleles. Conclusion: Apo E allele polymorphism is not an independent risk factor for type 2 diabetes complicated with coronary heart disease