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利用植物细胞工程技术及组织培养技术:①离体快繁含长春花碱及长春新碱高的长春花幼苗.长春花离体快繁诱导与芽增殖的培养基为:MS+2.4-Dlmg·L~(-1)(单位下同)+ZT1,蔗糖30g·L~(-1),琼脂粉4g·L~(-1),pH5.4～5.8.诱导生根培养基为1/2MS+2.4-D1+KT0.4,蔗糖20g·L~(-1),琼脂粉和 pH 值同芽诱导培养基.②长春花植物细胞悬浮系的建立.愈伤组织诱导培养基为 MS+2.4-D1,MS+NAA1.86.长春花的开发研制工作从工业和农业二方面立体进行。
Plant cell engineering and tissue culture techniques were used: (1) In vitro propagation of vinca elk and vincristine-tolerant vinca tuberosum seedlings in vitro was carried out with the medium of MS + 2.4-Dlmg · L -1 and units ZT1, sucrose 30 g · L -1, agar powder 4 g · L -1, pH 5.4 ~ 5.8. The rooting medium was 1 / 2MS + 2.4-D1 + KT 0.4, sucrose 20 g · L -1, agar powder and pH-induced culture medium.②The establishment of suspension cell of Catharanthus roseus L. The callus induction medium was MS + 2.4- D1, MS + NAA1.86. The development of vinca flowers from the industrial and agricultural aspects of three-dimensional.