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Objective:To study the inhibitory effect of Nordy on HPV16 E6 gene in human immortalized endocervical cells(H8). Methods:The expressions of E6 and p53 proteins of H8 cells were detected by immunocytochemistry,and the expression of HPV16E6 mRNA of H8 cells was detected by RT-PCR. Results:According to immunocytochemistry,expressions of E6 and p53 proteins of H8 cells after treatment with 100 μmol/L Nordy for 72 h were weaker than control group obviously.After treatment with 50,100,200 μmol/L Nordy for 72 h.HPV16E6 mRNA expressions of H8 cells were lower than the control group significantly(P＜0.05).Conclusion:Nordy could inhibit the expression of HPV16 E6 mRNA in H8 cells,influence transcription of E6 gene and result in decreased expressions of E6 and p53 proteins.