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ISSR是一种特异性较强、稳定性较高的分子标记方法。本研究采用ISSR-PCR技术,检测了红松(PinuskoraiensisSieb.etZucc)在伊春市汤旺河高峰林场、长白山二道白河、黑河胜山林场和俄罗斯海参崴市郊的四个种群的遗传多样性和遗传分化。15个引物的扩增结果表明:红松群体的多态位点比率是60.70%,平均每个引物3.6个多态位点,多样性水平在松科植物中是较高的;红松分布中心区的遗传多样性要高于边界区;和大多数木本植物一致,红松群体的基因多样性主要来自种群内部,占总基因多样性的73%;红松四个种群的遗传距离和地理距离之间无正相关性。根据研究结果推测,天然红松分布区逐渐缩小的原因不是由于遗传多样性水平过低引起的,而是由于人类的破坏作用,再加上火灾和风倒等因素造成的。
ISSR is a more specific and stable molecular marker method. In this study, genetic diversity and genetic diversity of four populations of Pinus koraiensis Sieb.etZucc in Gaofeng Forest Farm of Tangwang River, Erdaobai River in Changbai Mountains, Shengshan Forest Farm in Heihe River and the suburbs of Vladivostok in Russia were detected by ISSR-PCR. Differentiation. The amplification results of the 15 primers showed that the polymorphic locus ratio of P. koraiensis population was 60.70% with an average of 3.6 polymorphic loci per primer, and the diversity level was higher in the Pinaceae plants. The distribution of the Korean pine The genetic diversity of the district was higher than that of the border area. Consistent with most woody plants, the genetic diversity of the Korean pine population mainly came from within the population, accounting for 73% of the total. The genetic distance and geographical distribution of the four populations There is no positive correlation between the distances. According to the results of the study, it is speculated that the reason why the natural Korean pine is gradually reduced is not caused by the low level of genetic diversity, but due to the destructive effect of human beings and the factors of fire and wind collapse.