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采用高效液相色谱法测定海洛因的含量,色谱柱:Nova-pak C-(18)柱,流动相:0.05mol/L磷酸氢二钠-磷酸二氢钠:乙腈(80:20)(pH=6.9),流速:2.0ml/min,紫外检测波长为214nm,在海洛因浓度为2.93μg/ml-6000.00Pg/ml范围内,线性良好,回归方程为Y=0.026x+0.5204,r=0.9999,Y为峰面积(X10~6毫伏·s),x为浓度(Pg/ml)。海洛因标准品检测的日内平均标准偏差为1.42％～2.16％;日间平均偏差为2.05％～2.25％;平均加样回收率为100.45％,RSD为1.91％。采用此方法检测了5个批号的收缴海洛因的含量,分别为96.40％,94.34％,91.32％,35.85％,79.61％。
The content of heroin was determined by high performance liquid chromatography (HPLC). The column was Nova-pak C- (18). The mobile phase consisted of 0.05mol / L sodium phosphate dibasic sodium phosphate monobasic acetonitrile acetonitrile 80:20 (pH = 6.9), the flow rate was 2.0ml / min, the UV detection wavelength was 214nm, the linearity was good in the heroin concentration range of 2.93μg / ml-6000.00Pg / ml, the regression equation was Y = 0.026x + 0.5204, For the peak area (X10 ~ 6 mV · s), x is the concentration (Pg / ml). The average standard deviations of heroin standard detection ranged from 1.42% to 2.16%. The average daytime deviations ranged from 2.05% to 2.25%. The average recovery was 100.45% and the RSD was 1.91%. This method was used to detect the heroin seized by five batches, which were 96.40%, 94.34%, 91.32%, 35.85% and 79.61% respectively.