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目的：探讨利用同工酶技术对灵芝不同菌株的分类鉴定方法。方法：应用聚丙烯酰胺凝胶电泳法，分析比较过氧化物同工酶和酯酶同工酶酶谱的变化。结果：灵芝５个菌株间存在相似的酶带和相近的Ｒｆ值，亲缘关系极其相近，紫芝与其它菌株间酶谱差异较大，亲缘关系相对较远。结论：利用酯酶同工酶对灵芝菌株及菌种进行分类鉴定是一种可靠的方法
Objective: To explore the methods of classification and identification of different strains of Ganoderma lucidum using isoenzyme technology. Methods: Polyacrylamide gel electrophoresis was used to analyze the changes of isoenzymes of peroxidase isoenzyme and esterase isoenzyme. RESULTS: Similar bands and similar Rf values existed among the five strains of Ganoderma lucidum. The genetic relationship was very similar, and there was a big difference in the zymogram between G. lucidum and other strains. The genetic relationship was relatively distant. Conclusion: The use of esterase isoenzymes to classify and identify ganoderma lucidum strains and strains is a reliable method