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目的用ｍＲＮＡ差异显示技术观察ＳＨＲ与ＷＫＹ大鼠肾脏组织在ｍＲＮＡ表达水平上的差异。方法取１２周龄的ＳＨＲ及ＷＫＹ大鼠，提取肾脏总ＲＮＡ，用含１２个寡聚核苷酸的Ｔ１１Ａ或Ｔ１１Ｇ引物进行逆转录，用Ｔ１１Ａ、ＡＰ１～３，Ｔ１１Ｇ、ＡＰ６，Ｔ１１Ｇ、ＡＰ７～８引物对逆转录产物进行多聚酶链式反应，反应产物进行聚丙烯酰胺凝胶电泳及放射自显影。结果每一种引物组合在单一电泳泳道上可显示７０～１００条基因表达条带，其中绝大多数代表肾脏基因表达的条带是相同的，但其中显示的４条ＳＨＲ大鼠肾脏基因表达的条带与相对应的ＷＫＹ大鼠肾脏基因表达的条带出现了差异。讨论ｍＲＮＡ差异显示技术对于快速筛选ＳＨＲ及ＷＫＹ大鼠肾脏组织的差异表达基因是适用的。
Objective To observe the difference of mRNA expression between SHR and WKY rats by mRNA differential display. Methods Twelve - week - old SHR and WKY rats were used to extract total RNA from kidney and reverse transcribed with T11A or T11G primers containing 12 oligonucleotides. T11A, AP1 ~ 3, T11G, AP6, T11G, AP7 ~ 8 primers for reverse transcription products by polymerase chain reaction, the reaction product was polyacrylamide gel electrophoresis and autoradiography. Results Each primer combination showed 70-100 gene expression bands on a single electrophoresis lane. Most of the bands representing kidney gene expression were the same, but the expression of kidney gene in 4 SHR rats Bands and corresponding WKY rat kidney gene expression of the band difference. Discussion The mRNA differential display technique is suitable for the rapid screening of differentially expressed genes in kidney tissues of SHR and WKY rats.