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用聚丙烯酰胺包埋，再以戊二醛交联联合固定了α淀粉酶、糖化酶和葡萄糖异构酶，对联合固定化的最适条件和联合固定化酶的性质进行了初步研究．结果表明联合固定化酶较固定化单酶更能发挥协同效应，能够将底物一步水解并转化为产物．文中所用的固定化方法与其他方法相比，固定化酶的稳定性、半衰期及酶固定化后活力均较高．在６０℃、ｐＨ５５时，α淀粉酶和糖化酶二酶的联合固定化酶的活力最高，其操作半衰期达到７９ｈ；在６０℃、ｐＨ７５时，α淀粉酶、糖化酶和葡萄糖异构酶三酶的联合固定化酶的活力最高，其操作半衰期达到９０ｈ．
After being encapsulated with polyacrylamide, the α-amylase, glucoamylase and glucose isomerase were immobilized by cross-linking of glutaraldehyde. The optimal conditions for co-immobilization and the properties of co-immobilized enzyme were studied. The results showed that the combination of immobilized enzyme and immobilized single enzyme more synergistic effect, the substrate can be one-step hydrolysis and conversion to the product. Immobilized method used in this article compared with other methods, the stability of the immobilized enzyme, half-life and high activity after enzyme immobilization. At 60 ℃, pH55, α-amylase and saccharifying enzyme combined immobilized enzyme had the highest activity and its half-life reached 79h. At 60 ℃ and pH75, the activity of α-amylase, glucoamylase and glucose The activity of enzyme-linked immobilized enzyme was the highest, and its half-life was 90h.