论文部分内容阅读
DNA 重组技术引入乙型肝炎研究,使人类对乙型肝炎病毒(HBV)有了新的认识。1978年首次将 HBV DNA 克隆成功,标志着对 HBV 的研究重点从免疫学到分子生物学的战略性转移。一、HBV 基因结构已基本阐明HBV 的几个主要亚型均已有克隆,对全长3.2kb 的基因组已作了详尽的内切酶图谱分析,发现了 HBV 基因组的多态性,表明亚型之中仍有核苷酸顺序的差异。已经认识到 HBV 基因组可分为4个大的阅读框架(S、P、C 和 X),分别编码表面抗原(HBsAg)、核心抗原(HBcAg)、DNA 多聚酶及一个性质不明的 x 抗原
The introduction of DNA recombination technology into the study of hepatitis B, so that humans have a new understanding of hepatitis B virus (HBV). The first successful HBV DNA cloning in 1978 marked a strategic shift from immunology to molecular biology in the focus of HBV research. First, the HBV gene structure has been basically elucidated several major subtypes of HBV have been cloned on the full-length 3.2kb genome has done a detailed endonuclease pattern analysis and found the HBV genome polymorphism, indicating that the subtype There are still nucleotide sequence differences. It has been recognized that the HBV genome can be divided into four large reading frames (S, P, C and X), encoding the surface antigen (HBsAg), core antigen (HBcAg), DNA polymerase and an unidentified x antigen