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为了明确花瓣的伸展机制,以‘凤丹白’牡丹(Paeonia ostii)为研究对象,借助傅里叶变换红外光谱(Fourier Transformation Infrared Spectroscopy,FTIR)及高斯多峰拟合,分析蕾期、初开期和盛开期花瓣的波谱特征及蛋白质二级结构的差异性。主要结果如下:随着花朵开放花瓣伸展,777、1 105、1 155、1 243、1 447、1 651、1 740、2 853和2 926 cm~(-1)处的振动峰振动依次增强,表明磷脂质、核酸、脂类和蛋白质等代谢物质随花瓣的伸展逐渐增强;且蕾期1 030 cm~(-1)处的振动峰在花开后移至1 060 cm~(-1),表明蕾期花瓣细胞壁多糖以甘露聚糖为主,花朵开放后则以阿拉伯糖为主。蛋白质中甲基基团含量(A2 951 cm~(-1)/A 2 858 cm~(-1))出现平稳下降的趋势,初开期比蕾期减少7.97%,盛开期减少13.38%;糖蛋白的变化(A 1 083 cm~(-1)/A 1 547 cm~(-1))也呈现下降趋势,推测‘凤丹白’牡丹是通过蛋白质糖基化作用调控花瓣的伸展过程。对氨基Ⅰ区域(1 600~1 700 cm~(-1))高斯多峰拟合数据显示,各阶段花瓣中均含有果胶相连的β–折叠、β–折叠、无规卷曲、α–螺旋及环与转角;α–螺旋、β–折叠及无规卷曲所占比例在花瓣伸展过程中呈现增长趋势,表明蕾期花瓣通过降低α–螺旋应对缺水造成的生理胁迫,并可以利用ATP合成花瓣生长发育所需的物质;花瓣通过形成更多功能域较多的蛋白质,有效调控花瓣伸展时复杂的生物化学过程。
In order to clarify the stretch mechanism of petals, we used the Paeonia ostii (Paeonia ostii) as the research object and analyzed the effects of bud stage, early stage Spectral Characteristics and Protein Secondary Structure Differences of Petals in Periods and Blooming Periods. The main results are as follows: With the flower petals extended, the vibrational vibration at 777,1105,1 155,1 243,1 447,1 651,1 740,2853 and 2 926 cm -1 increased in turn, The results showed that the metabolites such as phospholipids, nucleic acids, lipids and proteins increased gradually with the extension of the petals. The vibrational peak at 1 030 cm -1 shifted to 1 060 cm -1 after flowering, The results showed that the budding cell wall polysaccharides were mainly mannan and the flowers were mainly arabinose after opening. The contents of methyl groups in protein (A2 951 cm -1 / A 2 858 cm -1) decreased steadily, decreasing by 7.97% at initial stage and 13.38% at blooming stage. The content of sugar The change of protein (A 1 083 cm -1 / A 1 547 cm -1) also showed a downward trend. It is speculated that ’Fengdanbai’ peony regulates petal stretch through protein glycosylation. The Gaussian multimodal fitting data for the amino group Ⅰ region (1 600 ~ 1 700 cm -1) showed that all the petals contained pectin-linked β-sheet, β-sheet, random coil, α-helix And the ring and corner. The proportion of α-helix, β-sheet and random coil showed an increasing trend during petal extension, indicating that petals of bud undergo physiological stress caused by water shortage by reducing α-helix and can utilize ATP synthesis Petals need to grow and develop the material; petals through the formation of more functional domains more protein, effectively control the complex petal stretch biochemical processes.