论文部分内容阅读
目的建立HPLC-MS/MS法测定人血浆中氢吗啡酮的质量浓度。方法用固相萃取法处理血浆,用Kromasil 100-5SIL和E97915色谱柱,以95%乙腈-5 mmol·L-1乙酸铵为流动相,梯度洗脱,用正离子、多反应监测方式测定样品质量浓度。结果血浆样品中,氢吗啡酮在0.05~10.00 ng·m L-1内线性关系良好(r=0.999 8),最低定量下限为0.05 ng·m L-1。血样日内与日间RSD均小于15%,平均回收率>70%,且稳定性均较好。结论本方法简便快速、灵敏准确、特异性强,适用于氢吗啡酮的体内药代动力学研究。
Objective To establish a HPLC-MS / MS method for the determination of hydromorphone in human plasma. Methods Plasma was processed by solid-phase extraction. The samples were eluted with Kromasil 100-5SIL and E97915 columns using 95% acetonitrile-5 mmol·L-1 ammonium acetate as mobile phase with gradient elution and positive ion and multi-reaction monitoring methods Concentration. Results The linearity of hydromorphone was 0.05 ~ 10.00 ng · m L-1 in plasma samples (r = 0.999 8). The lowest limit of quantitation was 0.05 ng · m L-1. The intra-day and interday RSDs of blood samples were all less than 15%, the average recovery was> 70%, and the stability was better. Conclusion This method is simple, rapid, accurate, sensitive and specific. It is suitable for in vivo pharmacokinetics study of hydromorphone.