复方芍红颗粒的薄层鉴别研究

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目的:建立复方芍红颗粒中四种药材的TLC鉴别法。方法鉴别白芍的TLC条件为:以三氯甲烷︰乙酸乙酯︰甲醇︰甲酸=10︰1.25︰3︰0.1为展开剂,喷5%香草醛硫酸溶液;鉴别红花的TLC条件为:以乙酸乙酯︰甲酸︰水︰甲醇=7︰2︰3︰0.4为展开剂;鉴别川芎的TLC条件为:以正己烷︰乙酸乙酯=5︰1为展开剂,在紫外365n m下观察;鉴别蔷薇红景天的T L C条件为:三氯甲烷-甲醇-丙酮-水(6︰3︰1︰1),用碘蒸气熏15-20分钟。结果在该鉴别条件下,各斑点清晰,分离度较好。结论该方法简便快捷,重复性好,专属性高,为复方芍红颗粒的质量控制提供参考。“,”Objective To identify the components in Fufang Shaohong granules. Methods The condition of TLC which was used to identify Paeonia lactiflora Pal is as folowing: the mixture of chloroform, ethyl acetate, methanol and formic acid (10︰1.25︰3︰0.1) is developing agent, and the 5% vanilin sulfuric acid solution is chromogenic agent. The condition of TLC which was used to identify Carthamus tinctorious is as folowing: the mixture of ethyl acetate, formic acid, water and methanol (7︰2︰3︰0.4) is developing agent. The condition of TLC which was used to identify the hawthorn is as folowing: the mixture of methylbenzene, ethyl acetate and formic acid (10︰2︰0.5) is developing agent. The condition of TLC which was used to identify the Rhodiola rosea L. is as folowing: the mixture of chloroform, methanol, acetone and water (6︰3︰1︰1) is developing agent, and the silica gel plat is placed under the Iodine vapor 15-20 minutes. Results there was a good separating degree and the spots were clear. Conclusion The method is simple, fast, repeatable and high specific, which can be used to control the quality of Fufang Shaohong granules.
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