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利用北京正负电子对撞机直线加速器E2束流打靶产生高能混合粒子场,以0、109、145、1952、84和560Gy的剂量处理两个冬小麦品种ZY9和ZH7,并与相同剂量的60Coγ射线相比较,研究其细胞学效应。试验结果表明,混合粒子场辐照小麦种子可抑制根尖细胞有丝分裂,诱发染色体出现单微核、双微核、多微核、环状染色体、落后染色体、游离染色体、染色体断片等多种畸变类型。混合粒子场与γ射线诱发的微核畸变率和染色体畸变率均存在明显的剂量效应,但混合粒子场的损伤效应明显高于γ射线。混合粒子场可诱发高频率的环状染色体和染色体断片,显示出混合粒子场处理小麦具有比γ射线处理更高的相对生物学效应。
High-energy hybrid particle fields were produced by beam-forcing of E2 electron beam at Beijing Electron-Positron Collider. Two wheat cultivars ZY9 and ZH7 were treated with doses of 0, 109, 145, 1952, 84 and 560 Gy, Compared to study its cytological effects. The results showed that irradiation of mixed wheat seeds could inhibit the mitosis of root tip cells and induce many types of aberrations such as single micronuclei, double micronuclei, multi-micronuclei, circular chromosomes, backward chromosomes, free chromosomes and chromosome fragments . There was a significant dose-effect in both the mixed particle field and γ-ray induced micronucleus aberration rate and chromosome aberration rate, but the damage effect of mixed particle field was significantly higher than that of γ-ray. The mixed particle field can induce high frequency circular chromosomes and chromosomal fragmentation, showing that mixed particle field treatment of wheat has a higher relative biological effect than gamma ray treatment.