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在室温条件下 ,阿的平能嵌入 DNA的碱基对之间 ,而阿的平荧光大幅猝灭 .酸性介质下 ,激发波长 348,4 2 8或 4 4 9nm,碱性介质下激发波长为 36 2或 4 2 2 nm,发射波长均为 5 0 2 nm.基于此对核酸进行定量测定 ,方法简单、快速、灵敏 .对于双链或单链小牛胸腺 DNA,线性范围 0～ 4μg/ m L,检测限 0 .0 3μg/ m L,共存物质干扰小 .在不同浓度 ct DNA存在下 ,温度对荧光猝灭的影响结果显示猝灭方式为静态猝灭
At room temperature, the level of a can be embedded between the base pairs of DNA, while the sharp fluorescence of a sharp quenching acid medium, the excitation wavelength 348,428 or 449nm, the excitation wavelength of the alkaline medium is 36 2 or 42 22 nm, and the emission wavelength is 502 nm.A quantitative method for nucleic acid quantification based on this method is simple, rapid and sensitive.For the double-stranded or single-stranded calf thymus DNA, the linear range is 0-4μg / m L, the detection limit of 0 3μg / m L, coexistence of material interference is small in the presence of different concentrations of ct DNA, the fluorescence quenching temperature quenching results show that the quenching mode is static quenching