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目的观察姜黄素对慢性脑缺血老龄鼠认知功能障碍和血清高密度脂蛋白(High density lipoprotein,HDL)水平的影响。方法采用双侧颈总动脉永久性结扎(Permanent occlusion of bilateral common carotid arteries,2VO)建立老龄鼠慢性脑缺血模型,并将模型鼠随机分为单纯缺血组以及姜黄素高剂量组(100 mg/kg)和低剂量组(50 mg/kg),另设假手术组和正常对照组,单纯缺血组、假手术组和正常对照组注射等量DMSO。各组均于术后24 h开始经腹腔注射,每日1次,连续30 d。采用Morris水迷宫行为学实验测试老龄鼠的空间学习及记忆功能,全自动生化分析仪检测血清HDL水平。结果给药30 d后,经连续5 d的训练,姜黄素高、低剂量组的逃避潜伏期均明显短于单纯缺血组(P<0.01),在原平台所在象限停留时间占总游泳时间的比例明显高于单纯缺血组(P<0.01或P<0.05),血清中HDL水平明显高于单纯缺血组(P<0.01)。结论姜黄素可以改善慢性脑缺血老龄鼠的认知功能障碍,并提高血清HDL水平。本实验为早期诊断血管性痴呆提供了新的生物学指标,也为姜黄素治疗血管性痴呆提供了新的药物靶点。
Objective To observe the effect of curcumin on cognitive dysfunction and serum high density lipoprotein (HDL) in aged rats with chronic cerebral ischemia. Methods Chronic cerebral ischemia model was established by permanent occlusion of bilateral common carotid arteries (2VO). The model rats were randomly divided into two groups: the pure ischemia group and the curcumin high dose group (100 mg / kg) and low dose group (50 mg / kg). Another sham-operated group and normal control group were injected with the same amount of DMSO as the normal ischemia group, the sham operation group and the normal control group. All groups were injected intraperitoneally 24 hours after operation, once a day for 30 days. Morris water maze behavioral test was used to test the spatial learning and memory function in aged rats. Serum HDL level was measured by automatic biochemical analyzer. Results After 30 d administration, the escape latency of curcumin high and low dose groups were significantly shorter than that of ischemia group (P <0.01) after training for 5 consecutive days. The proportion of stay time in the quadrant of the original platform to the total swimming time (P <0.01 or P <0.05), and the level of HDL in serum was significantly higher than that in ischemia group (P <0.01). Conclusion Curcumin can improve the cognitive dysfunction in aged rats with chronic cerebral ischemia and increase the level of serum HDL. This experiment provides a new biological index for the early diagnosis of vascular dementia and also provides a new drug target for the treatment of vascular dementia by curcumin.