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在美国,有免疫损害的卡氏肺孢子虫患者,在临床上常表现为弥漫性间质性肺炎。肺孢子虫感染占全部间质性肺炎病例的44%。因此,很有必要建立合适的实验诊断方法。作者用大鼠为模型,探讨了对包囊、孢子体或滋养体都可行的各种染色方法。按Frekel等法进行大鼠肺孢子虫感染。将24只Spraque-Dawley大鼠分成3组,第1组20鼠,在饮水中给予500ml/l的四环素,并每两周皮下注射25mg醋酸考的松1次;第2组2鼠,只给
In the United States, immunocompromised patients with Pneumocystis carinii often manifest as diffuse interstitial pneumonia in the clinic. Pneumocystis infections account for 44% of all cases of interstitial pneumonia. Therefore, it is necessary to establish a suitable experimental diagnostic method. The authors used the rat model to explore various methods of staining that are feasible for cysts, sporophytes or trophozoites. Pneumocystis carinii infection was performed by Frekel et al. Twenty-four Spraque-Dawley rats were divided into three groups: Group 1 20 rats given 500 ml / l tetracycline in drinking water and subcutaneous injection of 25 mg cortisone acetate every two weeks; group 2 rats given only