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目的观察仿刺参糖胺聚糖(HGAG)对环磷酰胺诱导的骨髓抑制贫血小鼠外周血及骨髓细胞周期的影响。方法将40只雄性昆明种小鼠随机分为正常组、模型组、HGAG治疗组(1,5,15mg·kg-1),每组8只。除正常组外其余各组腹腔注射环磷酰胺100mg·kg-1制备贫血小鼠模型,每天1次连续3d,并于造模当日起,HGAG给药组腹腔注射不同浓度的HGAG,正常组和模型组腹腔注射生理盐水,每天1次连续7d。用全自动血细胞分析仪、白细胞计数法、流式细胞术分别检测HGAG对贫血小鼠外周血、骨髓有核细胞数和骨髓细胞周期的影响。结果 HGAG低、中、高剂量组能显著升高外周血白细胞(WBC)和血红蛋白(HGB),且高剂量组还能明显升高外周血红细胞(RBC)和血小板(PLT)数量。HGAG 3个剂量组能显著增加小鼠骨髓有核细胞数和脾脏指数,而且能够促进骨髓G0/G1期细胞向S期及S期细胞向G2/M期细胞的转化,显著升高增殖指数。结论 HGAG能够解除细胞周期阻滞,促进骨髓造血细胞的增殖,增加外周血细胞、骨髓有核细胞的数量和升高脾脏指数,从而促进骨髓抑制贫血小鼠造血功能的恢复。
Objective To observe the effect of glycosaminoglycan (HGAG) on the cell cycle in peripheral blood and bone marrow of mice with myelosuppression induced by cyclophosphamide. Methods Forty male Kunming mice were randomly divided into normal group, model group and HGAG treatment group (1,5,15 mg · kg -1), with 8 mice in each group. Except the normal group, the other groups were given intraperitoneal injection of cyclophosphamide (100mg · kg-1) to establish a model of anemia mice once a day for 3 days. HGAG group was injected intraperitoneally with different concentrations of HGAG, normal group and The model group was intraperitoneally injected with normal saline once a day for 7 consecutive days. The effect of HGAG on the number of peripheral blood cells, the number of bone marrow nucleated cells and the cell cycle of bone marrow in anemia mice were detected by automatic hematology analyzer, white blood cell count and flow cytometry. Results HGAG low, medium and high dose groups could significantly increase WBC and HGB, and high dose group could significantly increase the number of peripheral blood red blood cells (RBC) and platelets (PLT). HGAG 3 dose group can significantly increase the number of bone marrow nucleated cells and spleen index of mice, but also promote bone marrow G0 / G1 phase cells to S phase and S phase cells to G2 / M phase cells, significantly increased proliferation index. Conclusion HGAG can relieve the cell cycle arrest, promote the proliferation of bone marrow hematopoietic cells, increase the number of peripheral blood cells, bone marrow nucleated cells and increase the spleen index, so as to promote the recovery of hematopoietic function in myelosuppressed mice.