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目的研究细胞因子诱导的杀伤细胞(CIK)联合顺铂对人肺腺癌细胞系A549的杀伤作用。方法在体外将健康者外周血单个核细胞(PBMC)用细胞因子诱导形成具有杀伤活性的CIK细胞。CIK、顺铂及CIK联合顺铂分别作用A549细胞24、48 h,MTT法检测细胞吸光度(A)值并计算各组细胞杀伤率。完全培养液培养CIK 48 h后,取上清液,按1∶1稀释后与A549细胞共培养24 h,用流式细胞仪检测A549细胞凋亡率。结果 CIK、顺铂对A549的杀伤活性呈时间、剂量依赖性(P<0.05)。5μg/mL顺铂联合效靶比为10∶1、20∶1的CIK共同作用A549细胞24 h,A549的杀伤率(%)分别为53.80±6.81,61.17±3.82,与单用顺铂及CIK相比,其对A549的杀伤率显著升高(P<0.05)。CIK上清液可诱导A549细胞凋亡。结论 CIK可增强顺铂对A549细胞系的杀伤作用。
Objective To study the killing effect of cytokine-induced killer (CIK) combined with cisplatin on human lung adenocarcinoma cell line A549. Methods Cytokines of peripheral blood mononuclear cells (PBMCs) of healthy individuals were induced to form cytotoxic CIK cells in vitro. A549 cells were treated with CIK, cisplatin and CIK plus cisplatin for 24 and 48 h respectively. The cell absorbance (A) was measured by MTT assay and cell killing rate was calculated. Complete culture medium CIK 48 h, the supernatant, diluted 1: 1 with A549 cells co-cultured for 24 h, flow cytometry A549 apoptosis rate. Results The killing activity of CIK and cisplatin to A549 was time-and dose-dependent (P <0.05). A549 cells were treated with 5μg / mL Cisplatin at a ratio of 10: 1 and 20: 1 for 24 h. The killing rates (%) of A549 cells were 53.80 ± 6.81 and 61.17 ± 3.82, respectively, Compared to its A549 killing rate was significantly increased (P <0.05). CIK supernatant can induce A549 cell apoptosis. Conclusion CIK can enhance the killing effect of cisplatin on A549 cell line.