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目的:研究克郁舒神颗粒对实验性恶劣心境肝气郁结证大鼠海马脑源性神经营养因子(BDNF)的影响。方法:选择60只雄性Wistar大鼠,随机分为空白组、模型组、克郁舒神颗粒高、中、低(1.50,0.75,0.375 g.kg-1)剂量组和氟西汀组,每组10只。采用慢性不可预见的温和性应激和孤养方法制备实验性恶劣心境肝气郁结证大鼠模型。应用免疫组织化学法检测大鼠海马BDNF蛋白表达水平。结果:与空白组比较,模型组大鼠大脑海马BDNF阳性神经元细胞明显减少,克郁舒神颗粒中、低剂量可以提高BDNF阳性神经元的面积比,优于高剂量组及氟西汀组(P<0.01);中、低剂量组之间差异无统计学意义。结论:脑组织海马区BDNF含量降低是导致恶劣心境发生的机制之一,克郁舒神颗粒能提高海马BDNF的含量,且疗效优于氟西汀。
Objective: To investigate the effect of “Keyu Shu Shen Granule” on hippocampus brain-derived neurotrophic factor (BDNF) in rats with experimental harsh mood and liver-qi stagnation syndrome. Methods: Sixty male Wistar rats were randomly divided into blank group, model group, Keyu Shu Shen Granule with high, medium and low doses (1.50,0.75,0.375 g.kg-1) and fluoxetine group Group of 10. Chronic unpredictable mild stress and loneliness methods were used to prepare rat model of experimental harsh liver qi stagnation syndrome. The expression of BDNF protein in hippocampus of rats was detected by immunohistochemistry. Results: Compared with the blank group, the BDNF positive neurons in the hippocampus of the model group were significantly decreased, while the low dose of Keyu Shu Shen Granules could increase the area ratio of BDNF positive neurons, which was better than the high dose and fluoxetine groups (P <0.01). There was no significant difference between the medium and low dose groups. CONCLUSION: Decreased BDNF content in hippocampus is one of the mechanisms leading to adverse mood. Hippocampus BDNF content can be increased by Keyu Shu Shen Granule, and the effect is better than fluoxetine.