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本实验在建立脊髓横断大鼠动物模型的基础上结合生化及电生理学等方法,观察了P物质(SP)对股四头肌肌电(EMG)发放的影响。结果表明,脊髓蛛网膜下腔注射(ith)辣椒素(cap)可以明显增强瘫痪肢体肌电的发放,而且这一作用可以被预先应用P物质抗血清(SPA)所阻断,ith SP也得到相似的结果;对横断脊髓前后脊髓前角和后角SP含量的测定发现,脊髓损伤后15天前角SP的含量没有明显改变,而后角的含量则明显增高;同时观察到下肢肌电发放对内源性P物质的敏感性也明显增加。结果提示,内源性P物质可能参与脊髓对下肢肌肉紧张度的调控。
In this study, we established the animal model of spinal cord transection on the basis of combined biochemical and electrophysiological methods to observe the substance P (SP) on the quadriceps EMG release. The results showed that injection of ith cap caplets into the spinal subarachnoid space could significantly enhance the electromyography of paralyzed limbs, and this effect could be blocked by P substance antiserum (SPA) in advance and ith SP also obtained Similar results; spinal cord anterior and posterior spinal cord transected and anterior horn angle SP content determination found that 15 days after spinal cord injury SP did not change the content of anterior horn, while the angle was significantly increased; the same time, the lower extremity EMG release was observed The sensitivity of endogenous substance P is also significantly increased. The results suggest that endogenous substance P may be involved in the regulation of spinal cord muscle tension in the lower extremities.