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The growth of 111 cultures of human epidermal keratinocytes inexplant culture,dispersed cell culture and 3T3 feeder layer culturewas studied.The rate of monolayer formation was 80.0% in 3T3feeder layer cultures,52.5% in explant cultures and only 11.8% indispersed cell cultures.It is concluded that among the modelsstudied the 3T3 feeder layer culture is the most effective andpossesses many advantages for the cultivation of human keratino-cytes;the explant culture would be a simple and easy method if wecan effectively control the contamination by fibroblasts.The growthpattern of cultured keratinocytes in each model was described.
The growth of 111 cultures of human epidermal keratinocytes in an inexplant culture, dispersed cell culture and 3T3 feeder layer culture was studied. The rate of monolayer formation was 80.0% in 3T3 feeder layer cultures, 52.5% in explant cultures and only 11.8% indispersed cell cultures. It is concluded that among the models that developed the 3T3 feeder layer culture is the most effective and postsses many advantages for the cultivation of human keratino-cytes; the explant culture would be simple and easy method if wecan effectively control the contamination by fibroblasts. growth pattern of cultured keratinocytes in each model was described.