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目的采用高效液相色谱-电喷雾质谱联用技术(LC-ESI-MS)对红车轴乙醇提取物中的化学成分进行分析鉴定,筛选红车轴中α-葡萄糖苷酶及黄嘌呤氧化酶抑制剂。方法选用反相C18色谱柱,流动相A为0.05%磷酸水溶液,流动相B为乙腈,流动相梯度程序:0~20 min,10%~55%B;流速0.8ml/min;样品进样量为10μl;检测波长:260 nm,利用电喷雾质谱(ESI-MS)负离子模式在线检测化学成分;以α-葡萄糖苷酶及黄嘌呤氧化酶作为生物靶分子,以超滤质谱技术筛选酶抑制剂。结果共鉴定出5个化学成分,分别为大豆素(daidzein),樱黄素(prunetin),染料木素(genistein),得鸢尾素(irilone)和山槐素(maackiain)。该5个化合物均可与黄嘌呤氧化酶亲和,除Gartanin外均可以与α-葡萄糖苷酶亲和。结论利用液质联用技术对红车轴中化学成分进行分析可以快速分析鉴定红车轴中的化合物并提供结构信息。利用超滤质谱技术筛选有效成分具有快速和灵敏等优势。
OBJECTIVE To analyze the chemical constituents of ethanol extracts of red axles by high performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS), select α-glucosidase and xanthine oxidase inhibitor . Methods The reversed-phase C18 column was used. The mobile phase A was 0.05% phosphoric acid solution and the mobile phase B was acetonitrile. The mobile phase gradient was 0-20 min, 10% -55% B, the flow rate was 0.8 ml / min. Was 10μl. The detection wavelength was 260 nm. The chemical constituents were detected online by electrospray mass spectrometry (ESI-MS). The α-glucosidase and xanthine oxidase were used as biological target molecules, and the enzyme inhibitors . As a result, five chemical constituents were identified, namely daidzein, prunetin, genistein, irilone and maackiain. The five compounds are both xanthine oxidase affinity, with the exception of Gartanin can be α-glucosidase affinity. Conclusions The analysis of chemical constituents in red axles using LC / MS can rapidly identify and identify compounds in red axles and provide structural information. The use of ultrafiltration mass spectrometry screening active ingredients has the advantages of fast and sensitive.