论文部分内容阅读
本试验以香艳梨的茎尖，不带芽茎段，带芽茎段，叶片，叶柄为试材，以１／２ＭＳ为基本培养基，分别附加０．５～２．０ｍｇ／Ｌ的６－卡基氨基嘌呤（６－ＢＡ）和０．１ｍｇ／Ｌ的萘乙酸（ＮＡＡ）、吲哚乙酸（ＩＡＡ），２，４－二氯苯氧乙酸（２，４－Ｄ）进行离体培养研究。结果表明，试材用０．１％ＨｇＣｌ２灭菌５～６分钟为宜；在７月份接种感染率低，愈伤组织形成较快；茎尖，不带芽茎段，带芽茎段，叶片均可作为离体培养的外植体，叶片的脱分化，分化的效果尤为明显，叶柄不宜作外植体；１／２ＭＳ＋１．５ｍｇ·Ｌ－１６－ＢＡ＋０．１ｍｇ·Ｌ－１ＮＡＡ有利于脱分化；１／２ＭＳ＋１．０ｍｇ·Ｌ－１６－ＢＡ有利于分化；瓶外生根优于瓶内生根。本试验可为香艳梨的工厂化育苗提供参考。
In this experiment, shoot tips, shoots with buds, shoots with buds, leaves and petioles of Xiangyan pear were used as test materials and 1 / 2MS medium was used as basal medium, and 0.5 ~ 2.0mg / L 6- In vitro study was carried out in vitro with 6-BA and NAA, IAA and 2,4-D (0.1 mg / L NAA) . The results showed that it was suitable to sterilize the test material with 0.1% HgCl2 for 5 to 6 minutes. The infection rate was low in July and the callus formed rapidly. The shoot tips, Which could be used as ex-vivo explants in vitro. The dedifferentiation and differentiation of leaves were particularly obvious. Petiole should not be used as explants. 1 / 2MS + 1.5mg · L-16-BA + 0.1mg · L-1NAA was beneficial to dedifferentiation ; 1 / 2MS + 1.0mg · L-16-BA is conducive to differentiation; rooting outside the bottle is better than the bottle rooting. This experiment may provide a reference for the plantation seedlings of Xiang-Yan pear.