论文部分内容阅读
通过花粉管通道介导银杏抗菌肽基因Gnk2-1于西瓜自交系‘04-1-2’中,以浓度为100、200、300和400 ng·μL-1的DNA为供体,分别于授粉后24、27、30和33 h导入,对子代进行PCR检测。结果表明:T0代坐果率和单瓜结籽数随授粉后处理时间的增加而升高,但在各个处理时间下均显著低于对照;外源DNA的导入对授粉后27 h处理的T1代种子发芽率和授粉后24 h处理的T1代种子出苗率均造成显著降低;不同DNA浓度转化的T1代种子的发芽率和出苗率与对照相比差异不显著;对1 280株T1幼苗进行PCR检测,得到32株阳性转化植株,总体转化率为2.5%,最佳转化时间为授粉后24~27 h,最佳DNA转化浓度为100~200 ng·μL-1;PCR阳性植株抗病鉴定表明转基因植株对西瓜枯萎病的抗性有所增强。
The Ginkgo biloba antifungal peptide gene Gnk2-1 was introduced into the watermelon inbred line ’04-1-2 ’via pollen tube channels. At concentrations of 100, 200, 300 and 400 ng · μL-1, At 24, 27, 30 and 33 h after pollination, PCR was performed on the offspring. The results showed that the rate of fruit set and the number of seeds per plant in T0 increased with the increase of pollination post-pollination time, but were significantly lower than that of the control at each treatment time. The seed germination rate and seed germination rate of T1 seedlings treated at 24 h after pollination all decreased significantly. The germination percentage and emergence rate of T1 seed seedlings transformed with different DNA concentrations were not significantly different from those of the control; 32 positive transformed plants were obtained, the overall conversion rate was 2.5%, the optimal transformation time was 24-27 h after pollination, and the optimal DNA transformation concentration was 100-200 ng · μL-1. The PCR-positive plants showed resistance Transgenic plants have increased resistance to watermelon blight.