论文部分内容阅读
作者认为存在于小鼠脾及其它淋巴样器官中的大颗粒淋巴细胞(LGL),具有自然杀伤(NK)活性及杀伤(K)活性。作者用2次不连续Percoll密度梯度离心法,于低密度部分发现富含NK及K活性的LGL,其形态学特征是在着色浅的胞浆中具有嗜天青颗粒和一个肾形核。LGL表面携带asialoGM_1抗原(一种以高浓度表达于小鼠NK细胞上的糖脂),用抗asialo GM_1 抗体可灭活NK及K活性,同时伴随着LGL的破坏。LGL表面缺乏可检出的Thy 1抗原,用抗Thy 1.2单克隆抗体处理后,NK活性虽有
The authors consider large granulocyte lymphocytes (LGLs) present in the spleen and other lymphoid organs of mice with both natural killer (NK) activity and killer (K) activity. Using two discontinuous Percoll density gradient centrifugation methods, the authors found that NKL and NK-rich LGL were found in low-density sections, and morphologically characterized by the presence of azurophilic granules and a reniform nucleus in a lightly-colored cytoplasm. The LGL surface carries the asialoGM 1 antigen, a glycolipid expressed at high concentrations on mouse NK cells, and inactivates NK and K activity with anti-asialo GM-1 antibodies with LGL destruction. LGL surface lack of detectable Thy 1 antigen, with anti-Thy 1.2 monoclonal antibody treatment, although NK activity