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目的:通过观察龟鹿二仙胶汤及其拆方对SD大鼠及豚鼠软骨细胞增殖的影响,比较在生理及病理状况下龟鹿二仙胶汤及拆方各药物的作用。方法:取1月龄SD大鼠和4月龄豚鼠膝关节软骨建立软骨细胞体外培养体系,运用MTT法比较龟鹿二仙胶及其拆方含药血清对不同软骨细胞增殖的影响。结果:中药血清各组对SD大鼠软骨细胞增殖均具有非常显著的促进作用,西药组明显高于中药各组;龟板与全方效果相当。中药血清各组对豚鼠软骨细胞增殖均具有非常显著的促进作用。龟鹿二仙胶、龟板促增殖效果与西药相比差异无统计学意义。人参的促增殖作用也比作用于正常软骨细胞时有所增强。结论:在病理状态下,龟鹿二仙胶、龟板的促增殖作用明显增强,人参在细胞机能状况下降时才明显发挥补虚作用。
OBJECTIVE: To observe the effects of Turtle Erxian Decoction and its disassembled formulas on the proliferation of chondrocytes in SD rats and guinea pigs, and to compare the effects of each compound of Gulu Erxian Glucose and its components under physiological and pathological conditions. Methods: Chondrocytes were cultured in vitro from the articular cartilage of 1-month-old SD rats and 4-month-old guinea pigs. MTT assay was used to compare the effects of Gulu Erxian gum and its drug-containing serum on the proliferation of different chondrocytes. Results: The serum of each group of traditional Chinese medicine on the proliferation of rat chondrocytes have a very significant role in promoting the Western medicine group was significantly higher than the Chinese medicine group; turtle shell and all the results quite. Chinese medicine serum groups of guinea pig chondrocytes proliferation have a very significant role in promoting. Guilu Erxian glue, turtle shell to promote proliferation and Western medicine compared with no significant difference. Proliferation of ginseng role also than normal chondrocytes increased. CONCLUSION: Under the pathological conditions, the promotion of proliferation of turtle Deer Immortelle and turtle shell was significantly enhanced, while ginseng showed a significant tonic effect when the cellular functional status decreased.