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目的：建立用高效液相色谱法测定血浆中泮托拉唑浓度的方法，为进行泮托拉唑的体内药物代谢、生物利用度研究提供基础。方法：血浆用０．０１ｍｏｌ·Ｌ－１磷酸氢二钠做ｐＨ调节剂，二氯甲烷做提取溶剂，硝西泮作内标。采用ＰｌａｔｉｎｕｍＣ１８（５ｕ，２５０ｍｍ×４．６ｍｍ）柱，流动相：甲醇水醋酸盐缓冲液（４０∶６０∶５），流速１．０ｍｌ·ｍｉｎ－１，检测波长２８６ｎｍ。结果：该法回收率平均在９８．３６％～１０４．２７％，重现性高，ＲＳＤ在４．６９３％～９．１２％范围内（ｎ＝５），线性范围：２０～４０００μｇ·Ｌ－１，最低检测浓度１５μｇ·Ｌ－１。结论：ＨＰＬＣ测定血浆中泮托拉唑的方法简便、准确、灵敏、专一，能满足血药浓度测定及药代动力学研究的需要。
OBJECTIVE: To establish a method for the determination of pantoprazole concentration in plasma by HPLC and to provide a basis for the study of the drug metabolism and bioavailability of pantoprazole in vivo. Methods: The plasma was treated with 0.01 mol·L-1 sodium phosphate dibasic, pH adjuster, methylene chloride as extraction solvent, and nitrazepine as internal standard. Platinum C18 (5u, 250mm × 4.6mm) column was used. The mobile phase was methanol-water-acetate buffer (40:60:5), the flow rate was 1.0ml · min-1 and the detection wavelength was 286nm. Results: The average recoveries of this method ranged from 98.36% to 104.27% with high reproducibility, RSD ranged from 4.693% to 9.12% (n = 5), linear range from 20 to 4000 μg · L -1, the lowest detection concentration of 15μg · L-1. Conclusion: The HPLC method for the determination of pantoprazole in plasma is simple, accurate, sensitive and specific. It can meet the need of determination of plasma concentration and pharmacokinetics.