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1973年奴卡菌胆固醇氧化酶(EC1.1.3.6简写 CO)开始被应用于临床胆固醇测定。次年胆固醇酯酶(EC3.1.1.13简写 CEH)与过氧化物酶(EC1.11.1.7简写 POD)被引用,成为较完整简便的酶法分析系统,为今后的发展奠定了基础。此项发展打破了近百年来胆固醇分析方法学上应用腐蚀性强酸的传统局面,增强特异性,简化方法。普遍应用报道较多的是酚一氨基安替比林一过氧化物酶显色系统,即 Trinder 反应
In 1973, slavone-resistant cholesterol oxidase (EC 1.1.3.6 abbreviated CO) was initially applied to clinical cholesterol determination. The next year, cholesterol esterase (EC 3.1.1.13 abbreviated CEH) and peroxidase (EC 1.1.1.7 abbreviated POD) were cited as a more complete and simple enzymatic analysis system, laying the foundation for future development. This development has broken the traditional situation of applying the corrosive strong acid in the method of cholesterol analysis for nearly a hundred years, enhancing the specificity and simplifying the method. More commonly reported is the application of phenol-amino antipyrine a peroxidase color system, the Trinder reaction