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背景 1990年以前,输血患者HCV感染常见,仅在法国输血后丙型肝炎病例超过20万例。现在分子技术可用于输血后丙型肝炎病毒感染的研究。研究设计和方法 从12对受-供血者获取了HCV包膜区分子序列,研究HCV株,属HCV1型(1a和1b亚型)、2型、3型、4型、5型。测定其遗传距离和突变率,并对照核苷数据库资料对基因遗传系统发生情况进行了序列分析。结果 经供-受血者配对,发现突变率小于0.05/位
Background Prior to 1990, HCV infection was common in transfusion patients, with more than 200,000 cases of hepatitis C after transfusions in France. Now molecular technology can be used for the study of hepatitis C virus infection after transfusion. Study Design and Methods HCV envelope regions were obtained from 12 pairs of recipients, and HCV strains of HCV1 (subtype 1a and 1b), type 2, type 3, type 4 and type 5 were studied. The genetic distance and mutation rate were determined, and the sequence analysis of the genetics system was carried out according to the nucleotide database. Results Paired by donor-recipient, the mutation rate was found to be less than 0.05 per locus