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目的探讨唾液HBV-DNA在乙型肝炎中的意义。方法采用荧光定量PCR法检测250例来新乡市中心医院就诊的HBV感染者的唾液和血清。结果250份标本血清中HBV-DNA为阴性的有25例(10.0%);唾液中HBV-DNA为阴性的有68例(27.2%)。血清中HBV-DNA大于103U/ml的有225例(90%),唾液中HBV-DNA大于103U/ml的有182例(72.8%)。其中大于105U/ml标本中,血清209例(83.6%),唾液123例(49.2%),血清与唾液HBV-DNA水平之间存在相关性(r=0.79,P<0.01)。结论乙型肝炎患者的唾液和血清中含有感染性的高载量HBV-DNA,可成为传染源之一。为乙型肝炎的流行病学研究提供了新的思路和理念。
Objective To investigate the significance of salivary HBV-DNA in hepatitis B patients. Methods Fluorescent quantitative PCR was used to detect the saliva and serum of 250 HBV infected patients who came to Xinxiang Central Hospital. Results 25 of 25 samples (10.0%) were negative for HBV-DNA in 250 samples and 68 (27.2%) were negative for HBV-DNA in saliva. There were 225 (90%) HBV-DNA> 103 U / ml in serum and 182 (72.8%) HBV-DNA in saliva more than 103 U / ml. There were 209 cases (83.6%) of sera and 123 cases of saliva (49.2%) in serum of more than 105 U / ml. There was a correlation between HBV-DNA level of serum and saliva (r = 0.79, P <0.01). Conclusion The infectious and high-load HBV-DNA in saliva and serum of patients with hepatitis B can be one of the sources of infection. Hepatitis B epidemiological research provides a new idea and philosophy.