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在获得稳定表达HLA G分子的K5 62细胞克隆的基础上 ,观察 4h及 16h不同效靶比例时NK细胞对靶细胞的杀伤效应 ,结果显示 ,NK细胞对转染HLA G基因的K5 62细胞的杀伤作用较未转染细胞明显降低 (P <0 0 1) ,用W6/ 32及BFL 1单克隆抗体阻断HLA G分子 ,可以恢复NK细胞的杀伤活性。用Y9/Z2 70及XA185 /Z2 70单抗特异性阻断抑制性受体CD94/NKG2A ,未观察到明显影响
On the basis of obtaining clone of K562 cell stably expressing HLA G, we observed the lethal effect of NK cells on target cells at different effective target ratios at 4h and 16h. The results showed that NK cells transfected K562 cells transfected with HLA G gene The killing effect was significantly lower than that of untransfected cells (P <0.01). The killing activity of NK cells was restored by blocking HLA G molecules with W6 / 32 and BFL1 monoclonal antibodies. Specific inhibition of the inhibitory receptor CD94 / NKG2A with Y9 / Z270 and XA185 / Z2 70 mAb was not observed