论文部分内容阅读
目的:探讨肾脏去神经对家兔动脉粥样硬化影响及其机制。方法:28只雄性新西兰白兔随机平均分为正常对照组(control)、肾脏去神经(renal denervation,RDN)+高脂组、假手术(sham)+高脂组、单纯高脂饮食(high-fat-diet,HFD)组。检测血脂、血浆去甲肾上腺素(norepinephrine,NE)和氧化低密度脂蛋白(oxidized low density lipoprotein,ox-LDL)浓度,免疫组化检测血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)和血小板内皮细胞粘附分子-1(platelet endothelial cell adhesion molecule-1,PECAM-1)的表达;Western blot检测细胞间粘附分子-1(intercellular cell adhesion molecule,ICAM-1)和血管细胞间粘附分子-1(vascular cell adhesion molecule,VCAM-1)的表达;RT-PCR检测MCP-1、PECAM-1、ICAM-1和VCAM-1的m RNA的表达。结果:术后RDN组NE水平低于sham组和HFD组(P<0.05),其血浆甘油三酯(triglyceride,TG)浓度低于HFD组(P<0.05);RDN组血浆ox-LDL浓度、主动脉的AngⅡ和粘附分子表达较sham组和HFD组明显降低(P<0.05),RDN组内膜/中膜比值较HFD组降低(P<0.05)。结论:RDN可降低TG浓度,同时可能通过降低交感神经活性,下调粘附分子的表达,延缓动脉粥样硬化形成。
Objective: To investigate the effect of renal denervation on atherosclerosis in rabbits and its mechanism. Methods: 28 male New Zealand white rabbits were randomly divided into control group, renal denervation (RDN) + hyperlipidemia group, sham + hyperlipidemia group, high- fat-diet, HFD) group. The plasma concentrations of norepinephrine (NE) and oxidized low density lipoprotein (ox-LDL) were measured. The expressions of angiotensin Ⅱ (AngⅡ), chemotaxis of monocytes The expression of monocyte chemoattractant protein-1 (MCP-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1) The expression of MCP-1, PECAM-1, ICAM-1 and VCAM-1 were detected by RT-PCR, and the expressions of ICAM-1, ICAM-1 and VCAM- M RNA expression. Results: The level of NE in RDN group was lower than that in sham group and HFD group (P <0.05), and the concentration of triglyceride (TG) in RDN group was lower than that in HFD group (P <0.05) The expressions of Ang Ⅱ and adhesion molecules in the aorta were significantly lower than those in sham group and HFD group (P <0.05). The intima / media ratio of RDN group was lower than that of HFD group (P <0.05). Conclusion: RDN can decrease TG concentration and delay the formation of atherosclerosis by decreasing the sympathetic activity, decreasing the expression of adhesion molecules.