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探讨了不同连接物质对顺铂(cis-dichlorodiamineplatinum,CDDP)与磁性纳米颗粒(magneticnanometerparticles,MNP)形成复合物的影响及所形成CDDP-MNP的特性.分别采用高分子海藻酸钠(sodiumalginate,SA)和小分子油酰肌氨酸(N-oleoylsarcosine,NOSARK)作为CDDP与磁性纳米颗粒的连接物,并与以羧甲基葡聚糖(carboxymethyldextran,CM-dex)为载体的CDDP复合物作对照,用透射电子显微镜、光子相关光谱(PCS)法、分光光度法等测定CDDP-MNP的理化特征,并通过载药量和稳定性的比较来评价两种分子量不同的连接物的优劣.透射电子显微镜下SA改性的CDDP-MNP呈基本均匀的圆球体,PCS直径分布约为43~52nm,磁核平均粒径为(8.8±1.3)nm.SA的载药量大于NOSARK,与CM-dex相当.以溶液形式保存在4℃条件下40d后,SA改性的CDDP-MNP复合物仍然稳定.结果表明,SA作为CDDP与磁性纳米颗粒结合的修饰物明显优于NOSARK,天然的SA可替代CM-dex作为CDDP与磁性纳米颗粒的连接物.实际应用时则要同时考虑最大可逆荷CDDP量和可逆结合的CDDP的利用率.
The effects of different linking substances on the formation of complexes of cis-dichlorodiamineplatinum (CDDP) and magneticnanometerparticles (MNPs) and the properties of CDDP-MNP formed by the two conjugates were investigated by using sodiumalginate (SA) And small molecule N-oleoylsarcosine (NOSARK) were used as the linker of CDDP with magnetic nanoparticles and compared with CDDP complex with carboxymethyldextran (CM-dex) as carrier. The physical and chemical characteristics of CDDP-MNP were determined by transmission electron microscopy, photon correlation spectroscopy (PCS) and spectrophotometry, and the advantages and disadvantages of two kinds of conjugates with different molecular weights were evaluated by drug loading and stability comparison. Under the microscope, the SA-modified CDDP-MNP showed a spherical shape with uniform diameter of 43 ~ 52nm and an average particle diameter of (8.8 ± 1.3) nm. The drug loading of SA was higher than that of NOSARK, The SA-modified CDDP-MNP complex remained stable after being stored in solution at 4 ° C for 40 days.The results show that the SA-modified CDDP as a magnetic nanoparticle-binding modifier is significantly better than NOSARK and the native SA is an alternative CM-dex as CDDP with magnetism Nanoparticle linker when considering practical applications will have maximum utilization reversible charge and reversible binding of CDDP amount of CDDP.