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目的:研究槲皮素对人前列腺癌PC-3细胞的凋亡作用。方法:体外培养PC-3细胞,给予不同浓度(50、100、150、200、250μmol/L)的槲皮素处理,MTT法检测槲皮素对细胞抑制率;流式细胞仪检测细胞凋亡率;透射电镜观察细胞超微结构的变化。结果:槲皮素能抑制人前列腺癌PC-3细胞的体外生长,且呈时间与剂量依赖性,浓度由低到高,其24 h的抑制率(%)分别为3.01±1.32、4.84±1.73、20.35±1.30、16.78±1.89、27.25±4.01,48 h的抑制率(%)分别为10.18±1.16、6.22±0.04、24.29±4.19、22.4±4.26、41.42±5.43,当药物浓度>150μmol/L时P<0.05,具有统计学意义;流式细胞术显示PC-3细胞凋亡率随槲皮素浓度升高和作用时间延长而增加(P<0.05),其中浓度150μmol/L和200μmol/L组,24 h的凋亡率(%)分别19.10±0.28、26.55±0.78,48 h的凋亡率(%)分别为27.65±1.06、38.30±5.96;电镜观察到细胞的典型凋亡形态学变化。结论:在适当的条件下,槲皮素对人前列腺癌PC-3细胞增殖有明显的抑制作用,同时可诱导PC-3细胞凋亡,其具体作用机制有待进一步深入研究。
Objective: To study the effect of quercetin on the apoptosis of human prostate cancer PC-3 cells. Methods: PC-3 cells were cultured in vitro and treated with quercetin at different concentrations (50, 100, 150, 200 and 250μmol / L). The inhibitory rates of quercetin were determined by MTT assay. Apoptosis was detected by flow cytometry Rate; TEM observation of changes in cell ultrastructure. Results: Quercetin inhibited the growth of human prostate cancer PC-3 cells in vitro and in a time-and dose-dependent manner. The concentrations of quercetin from low to high at 24 h were 3.01 ± 1.32 and 4.84 ± 1.73 , 20.35 ± 1.30,16.78 ± 1.89,27.25 ± 4.01,48 h respectively, the inhibitory rates (%) were 10.18 ± 1.16,6.22 ± 0.04,24.29 ± 4.19,22.4 ± 4.26,41.42 ± 5.43, respectively. When the drug concentration was> 150μmol / L (P <0.05). The results of flow cytometry showed that the apoptosis rate of PC-3 cells increased with the increase of quercetin concentration and the action time (P <0.05). The concentration of 150μmol / L and 200μmol / L The apoptotic rates (%) at 24 h were 19.10 ± 0.28,26.55 ± 0.78, respectively. The apoptotic rates (%) were 27.65 ± 1.06,38.30 ± 5.96 at 48 h, respectively. The typical apoptosis morphological changes were observed under electron microscope . CONCLUSION: Quercetin can significantly inhibit the proliferation of human prostate cancer PC-3 cells and induce the apoptosis of PC-3 cells under appropriate conditions. The specific mechanism remains to be further studied.