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Aim:The resurgence of severe acute respiratory syndrome(SARS)is still a threatbecause the causative agent remaining in animal reservoirs is not fully understood,and sporadic cases continue to be reported.Developing high titers of anti-SARShyperimmune globulin to provide an alternative pathway for emergent future pre-vention and treatment of SARS.Methods:SARS coronavims(CoV)F69(AY313906)and Z2-Y3(AY394989)were isolated and identified from 2 different Cantoneseonset SARS patients.Immunogen was prepared from SARS-CoV F69 strain.Sixhealth horses were immunized 4 times and serum was collected periodically tomeasure the profile of specific IgG and neutralizing antibodies using indirect en-zyme-linked immunosorbent assay and a microneutralization test.Sera were col-lected in large amounts at the peak,where IgG was precipitated using ammoniumsulphate and subsequently digested with pepsin.The product was then purifiedusing anion-exchange chromatography to obtain F(ab′)2 fragments,Results:Thespecific IgG and neutralizing antibody titers peaked at approximately week 7 afterthe first immunization,with a maximum value of 1:14210.The sera collected at thepeak were then purified.Fragment of approximately 15 g F(ab′)2 was obtained from1 litre antiserum and the purity was above 90% with the titer of 1:5120,which couldneutralize the other strain(SARS-CoV Z2-Y3)as well.Conclusion:This researchprovides a viable strategy for the prevention and treatment of SARS coronavirusinfection with equine hyperimmune globulin,with the purpose of combating anyresurgence of SARS.
Aim: The resurgence of severe acute respiratory syndrome (SARS) is still a threatbecause the causative agent remaining in animal reservoirs is not fully understood, and sporadic cases continue to be reported. Developing high titers of anti-SARS hyperimmune globulin to provide an alternative pathway for Emergent future pre-vention and treatment of SARS. Methods: SARS coronavims (CoV) F69 (AY313906) and Z2-Y3 (AY394989) were isolated and identified from 2 different Cantoneseonset SARS patients. Immunogen was prepared from SARS- CoV F69 strain. Sixth Health horses were immunized 4 times and serum was collected periodically to measure the profile of specific IgG and neutralizing antibodies using indirect en-zyme-linked immunosorbent assay and a microneutralization test. Sera were col-lected in large amounts at the peak, where IgG was precipitated using ammoniumsulphate and subsequently digested with pepsin. The product was then purified by anion-exchange chromatography to obtain F (ab ’) 2 fragments, Results: Thes pecific IgG and neutralizing antibody titers peaked at approximately week 7 afterthe first immunization, with a maximum value of 1: 14210. The sera collected at thepeak were then purified. Fragment of approximately 15 g F (ab ’) 2 was obtained from1 liter antiserum and the purity was above 90% with the titer of 1: 5120, which could not neutralize the other strain (SARS-CoV Z2-Y3) as well. Conlusion: This research project aids in prevention and treatment of SARS coronavirus infection with equine hyperimmune globulin, with the purpose of combating anyresurgence of SARS.