Isolation of Trichoderma reesei pyrG Negative Mutant by UV Mutagenesis and Its Application in Transf

• 作 者: LONG Hao WANG Tian-hong ZHANG
• 机 构: StateKeyLaboratoryofMicrobialTechnology
• 出 处: 高等学校化学研究：英文版
• 发表日期: 2008年5期
• 关键词: 基因突变体 生物化学 生菌散 杀菌剂 Trichoderma reesei UV mutagenesis pyrG negative strain pyrG-b
• 基金项目: Supported by the National Natural Science Foundation of China（No.30470052）, the National Basic Research Program Of China（Nos.2003CB716006 and 2004CB719702） and the Natural Science Research Foundation

Two uridine auxotrophic mutants of Trichoderma reesei were isolated by resistance to 5-fluoroorotic acid after UV mutagenesis. One mutant, called M23, was complemented with the Aspergillus niger pyrG gene carried by plasmid pAB4-1. A mutated pyrG gene of