【摘 要】
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In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reactio
【机 构】
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College of Life Sciences, Northeast Agricultural University, Harbin 1500300, China
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In this study, we used RAPD to analyze four kinds of color-flowered Salvia splendens Ker-Gawl, and the optimal RAPD reaction conditions were the optimal reaction mixture (25 uL total volume) that contained 2.0 μL 10×buffer,0.45 mmol·L-1 dNTPs, 2.0 mmol·L-1 Mg2+, 2 U Taq DNA polymerase, 0.30 umol·L-1 primer and 40 ng genomic DNA. Total 84 bands were amplified from 12 primers used, and the differential bands had 28 bands, which was 33% of total bands. In cluster group analysis, the four kinds of color-flowered were divided into two styles. One style is that the red color and red-white color were grouped together, then they grouped with purple color into one cluster, and the white color was another style.
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