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用膜片钳技术对比分析了棉铃虫三氟氯氰菊酯抗性品系 (R)及其同源对照品系 (S)幼虫离体培养中枢神经细胞Na+通道的门控特性及杀虫剂对R和S神经细胞Na+、Ca2 +通道门控过程的影响。结果表明 ,S神经细胞Na+通道电流 (S -INa)在 -50～ -40mV激活 ,-20mV左右达峰值 ,R神经细胞Na+通道电流 (R -INa)在-40mV左右激活 ,-10～0mV达峰值 ,即R -INa 激活电压与峰值电压均向正电位方向移动约10mV ,提示二者Na+通道门控特性不同 ,R神经细胞Na+通道功能发生了变异。三氟氯氰菊酯作用后 ,S -INa 与R -INa 的I～V曲线均向负电位方向移动约10mV ,S -INa 在20min后基本消失 ,而R -INa 被阻断需时约90min ,延长近5倍 ,其幅值有减小后再增大的现象。对Ca2 +通道分析表明 ,杀虫剂作用后 ,R及S神经细胞Ca2 +通道电流的I～V曲线均向负电位方向移动10～20mV ,提示三氟氯氰菊酯对Ca2 +通道的门控过程也有影响。与R -INa 幅值起伏变化相联系 ,可推知杀虫剂对神经细胞的毒性作用中 ,Na+、Ca2 +通道均受影响
The patch-clamp technique was used to compare the gating characteristics of Na + channel in vitro cultured CNS cells of cytoplasm (R) and its homologous control (S) larvae of Helicoverpa armigera and the effects of insecticides on R and S nerve Effect of Na + and Ca2 + channel gating on cell. The results showed that the Na + channel current (S-INa) of S neurons was activated at -50 ~ -40mV and peaked at -20mV, the Na + channel current (R-INa) of R neurons was activated at -40mV, Peak, that is R -INa activation voltage and peak voltage are positive potential direction of movement of about 10mV, suggesting that Na + channel gating characteristics of two different, R nerve cell Na + channel function mutation. After cyhalothrin, the I ~ V curves of S-INa and R-INa shifted to the negative potential about 10mV, S -INa disappeared after 20min, while R-INa was blocked for about 90min, 5 times its amplitude decreases and then increase the phenomenon. The analysis of Ca2 + channels showed that the I ~ V curves of Ca2 + channel currents of R and S neurons moved 10 ~ 20mV to the negative potential direction after insecticide action, suggesting that the cyhalothrin’s Ca2 + channel gating process also had influences. Associated with the fluctuation of amplitude of R -INa, it can be deduced that the toxicity of insecticides to nerve cells, Na +, Ca2 + channels are affected