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背景:作者前期实验以左室内压及其最大升降速率等8项心肌力学与血流动力学参数为指标,观察到人参二醇和三醇组皂甙均能使离体大鼠工作心脏收缩性能减弱。目的:探讨人参皂甙单体Rb1对大鼠心率、动脉压、左室内压(leftven-tricularpressure,LVP)、左室舒末压(leftventricularend-diastolicpres-sure,LVEDP)、室内压最大上升与最大下降速率(maximalrisingandfallingrateofventricularpressure,±dp/dtmax)6项指标的影响。设计:完全随机、空白对照的双盲法实验。地点、材料和干预:在吉林大学基础医学院动物实验室完成本实验,室温200C。Wistar大鼠购于吉林大学动物部,雌雄不拘。按随机抽签法将动物分为5组,即对照组、Rb195mg/kg组、Rb1190mg/kg组、Rb1285mg/kg组、维拉帕米(异搏定)36mg/kg组。在Wistar大鼠在体心脏上观察给予大鼠Rb195,190,285mg/kg,维拉帕米36mg/kg对大鼠心率、动脉压、LVP、LVEDP,+dp/dtmax,-dp/dtmax6项指标的影响,并与对照组进行比较。整个过程由高级实验师操作完成。主要观察指标:静脉注入维拉帕米与95,190,285mg/kg浓度Rb1后大鼠心率、动脉压、LVP、LVEDP,+dp/dtmax,-dp/dtmax6项指标的比较结果。结果:Rb1浓度在95,190,285mg/kg范围内均使心率、动脉压、左室收缩压、左室舒张压、室内?
BACKGROUND: In the previous experiments, the authors used 8 items of myocardial mechanics and hemodynamic parameters such as left ventricular pressure and its maximum lifting rate as indicators. It was observed that both panaxadiol and triol saponins can reduce the contractility of isolated working hearts in isolated rats. Objective: To investigate the effects of ginsenoside Rb1 on rat heart rate, arterial pressure, left ventricular pressure (LVP), left ventricular end-diastolic pressure (LVEDP), maximum increase in ventricular pressure, and maximum rate of decline (maximalrisingandfallingrateofventricularpressure, ±dp/dtmax) The effect of 6 indicators. Design: A completely randomized, controlled, double-blind experiment. Location, Materials, and Interventions: This experiment was performed at the Animal Laboratory of the Basic Medical College of Jilin University, room temperature 200C. Wistar rats were purchased from the animal department of Jilin University, either male or female. Animals were randomly divided into 5 groups: control group, Rb195mg/kg group, Rb1190mg/kg group, Rb1285mg/kg group, Verapamil (Verapamil) 36mg/kg group. Rats with heart rate, arterial pressure, LVP, LVEDP, +dp/dtmax and -dp/dtmax were observed with rat Rb 195,190,285 mg/kg and verapamil 36 mg/kg on Wistar rats. Influence and compare with the control group. The entire process is completed by a senior laboratory technician. MAIN OUTCOME MEASURES: Comparing the rat’s heart rate, arterial pressure, LVP, LVEDP, +dp/dtmax, and -dp/dtmax after intravenous injection of verapamil at a concentration of 95,190,285 mg/kg Rb1. Results: Rb1 concentrations in the range of 95,190,285 mg/kg all resulted in heart rate, arterial pressure, left ventricular systolic pressure, left ventricular diastolic pressure, and room?