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对外加脉冲电场处理的人红血球冷冻断裂和蚀刻的复型观察中发现在强电场(3KV/cm)作用下,细胞周围有颗粒状和纤维状结构。结合SDS电泳分析证明了它们是由于在电场作用下,红血球膜的带3蛋白和膜骨架蛋白(血影蛋白)脱出的结果。在强电场作用下,由于膜蛋白和膜骨架蛋白的脱出造成了对细胞膜的损伤,使细胞膜稳定性降低,细胞易变形和形成伪足。由于膜蛋白的脱出,多余的自由脂质进入细胞质内而形成泡状结构。外电场改变了蛋白-蛋白以及蛋白-脂分子间的作用可能是电穿孔的主要机理。本文还对当前公认的冷冻断裂中所观察到的膜中间颗粒的来源提出了疑问,并提出了它们还可能与冰晶有关。而冰晶的形成又与膜的亲水与疏水性有关。
In addition, the red blood cells treated by pulsed electric field were observed in cryogenic rupture and etching. The granular and fibrous structures around the cells were observed under the action of strong electric field (3KV / cm). Combined with SDS electrophoresis analysis proved that they are due to the electric field, the red blood cell membrane protein 3 and membrane scaffold protein (spectrin) prolapse results. Under the action of strong electric field, the cell membrane is damaged due to the release of membrane protein and membrane scaffold protein, the stability of the cell membrane is reduced, and the cells are easy to deform and form pseudopodia. Due to the prolapse of the membrane protein, excess free lipids enter the cytoplasm to form a vesicular structure. Changes in the external electric field protein-protein and protein-lipid interactions may be the main mechanism of electroporation. The paper also questions the currently recognized sources of membrane intergranular particles observed in cryogenic fractures and suggests that they may also be related to ice crystals. The formation of ice crystals and hydrophilic and hydrophobic membrane-related.