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目的研究海南地不容总生物碱的镇痛抗炎作用及急性毒性。方法采用小鼠热板法、扭体法及甲醛测痛试验观察镇痛作用;采用二甲苯致小鼠耳肿胀、醋酸致小鼠腹腔毛细血管通透性、棉球诱导小鼠肉芽组织增生试验模型观察抗炎作用,通过胸腔白细胞游走试验研究其对炎症渗出液中白细胞数量的影响,应用交叉菜胶致足肿胀试验测定炎性组织中前列腺素E(2PGE2)含量并初步探讨其抗炎机理,急性毒性试验采用改良寇氏法测定LD50。结果海南地不容总碱能明显延长小鼠舔后足时间,减少扭体次数,降低甲醛测痛试验第一、二时相的疼痛强度;减轻小鼠耳肿胀,抑制毛细血管通透性增高及肉芽组织增生,有效抑制白细胞游走,并能明显减少大鼠炎症组织中PGE2含量。其LD50为0.569g.kg-1,95%可信限为0.519～0.623g.kg-1。结论海南地不容总碱镇痛、抗炎作用显著,其机理可能与抑制PGE2的产生与释放有关。
Objective To study the analgesic and anti-inflammatory effects and acute toxicity of total alkaloids from Hainan. Methods The analgesic effect was observed by hot plate method, writhing method and formaldehyde test. The xylene-induced eardrum swelling, peritoneal capillary permeability of mice induced by acetic acid, granulation tissue hyperplasia induced by cotton balls The model was used to observe the anti-inflammatory effect. The leukocyte migration test was used to study the effect of interleukin on the number of leukocytes in inflammatory exudate. The content of prostaglandin E (2PGE2) Mechanism of inflammation, acute toxicity test Determination of LD50 by improved koji method. Results Total alkaloids in Hainan could significantly prolong the time of licking the hind foot, reduce the number of writhing, reduce the pain intensity in the first and second phases of the formaldehyde test, reduce the swelling of the ear, inhibit the increase of capillary permeability and Granulation tissue proliferation, effectively inhibit leukocyte migration, and can significantly reduce the inflammatory tissue PGE2 content. The LD50 was 0.569g.kg-1, and the 95% confidence limit was 0.519 ~ 0.623g.kg-1. Conclusion Hainan can not tolerate total alkaloids analgesic, anti-inflammatory effect was significant, the mechanism may be related to the inhibition of PGE2 production and release.