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为研究亚硒酸钠对人外周血淋巴细胞的致突变和抗诱变作用,调整人类淋巴母(TK6)细胞密度为1×106/ml,致突变试验分别用0.01、0.1、1、10、20、40μg/ml亚硒酸钠单独处理TK6细胞48 h;抗诱变性试验分别用0.01、0.1、1、10、20、40μg/ml亚硒酸钠+阳性致突变物MMC(0.05μg/ml)联合处理TK6细胞48 h。并测定细胞微核率。结果显示,20、40μg/ml亚硒酸钠染毒48 h后TK6细胞的微核率高于溶剂对照组,差异有统计学意义(P<0.05)。且随着亚硒酸钠染毒浓度的升高,TK6细胞的微核率呈上升趋势。0.01、0.1、20、40μg/ml亚硒酸钠+MMC染毒组TK6细胞的微核率均高于抗诱变组,差异有统计学意义(P<0.05);1、10μg/ml亚硒酸钠+MMC染毒组微核率均低于阳性对照组,差异有统计学意义(P<0.05)。且随着亚硒酸钠染毒浓度的升高,亚硒酸钠+MMC染毒组TK6细胞的微核率呈先下降后上升的趋势。提示亚硒酸钠在一定浓度(1～10μg/ml)下具有抗诱变性作用;而过高浓度亚硒酸钠(20～40μg/ml)具有致突变作用。
In order to study the mutagenesis and anti-mutagenic effects of sodium selenite on human peripheral blood lymphocytes, the density of human lymphoblastoid (TK6) cells was adjusted to 1 × 106 / ml. Mutagenicity experiments were performed with 0.01, 0.1, 1, The TK6 cells were treated with 20, 40μg / ml sodium selenite for 48 h. The anti-mutagenicity test was carried out with 0.01, 0.1, 1, 10, 20, 40μg / ml sodium selenite + positive mutagenic MMC (0.05μg / ml) combined with TK6 cells for 48 h. And measure the rate of cell micronucleus. The results showed that the micronucleus rate of TK6 cells in 20, 40μg / ml sodium selenite 48h was higher than that in solvent control group, the difference was statistically significant (P <0.05). And with the concentration of sodium selenite increased, the micronucleus rate of TK6 cells showed an upward trend. The micronucleus rates of TK6 cells in 0.01, 0.1, 20, 40μg / ml sodium selenite + MMC groups were significantly higher than those in the mutagens group (P <0.05) The rates of micronucleus in sodium acid + MMC group were lower than those in positive control group, the difference was statistically significant (P <0.05). With the increase of sodium selenite concentration, the micronucleus rate of TK6 cells in sodium selenite + MMC group first decreased and then increased. It suggested that sodium selenite had mutagenicity effect at a certain concentration (1 ~ 10μg / ml), while over-concentration of sodium selenite (20 ~ 40μg / ml) had mutagenic effect.