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进入21世纪,硝酸盐氮氧同位素测试技术的显著进展是反硝化细菌法测试技术的建立。反硝化细菌法具有可同时分析浓度低至μg/L的硝酸盐氮氧同位素组成、免去复杂的样品预处理、分析时间大大缩短以及从复杂溶液中只转化NO3-为N2O等优点。实验过程由细菌挑选和培养、样品NO3-转化为N2O、N2O提取纯化、同位素测试以及同位素测试结果校正等几个关键步骤组成。国外研究结果显示该技术不仅可用于海水和淡水中硝酸盐同位素测试,而且还可用于土壤样品,并得到不断的完善和应用;国内建立了这一技术,在包气带和地下水硝酸盐污染研究中取得重要进展。由于该技术诸多优点,建议大力促进这一技术在我国的研究和应用,希望有关部门加大支持力度,进一步完善该技术并推广应用。
In the 21st century, the significant progress of nitrate nitrogen and oxygen isotope testing technology is the establishment of denitrification method. The denitrifying bacteria method has the advantages of simultaneous analysis of nitrate nitrogen and oxygen isotopes at concentrations as low as μg / L, eliminating the need for complex sample preparation, greatly reducing the analysis time, and converting only the NO3- to N2O from complex solutions. The experiment consisted of several key steps of bacterial selection and culture, sample NO3- conversion to N2O, extraction and purification of N2O, isotope test and calibration of isotope test results. The results of foreign studies show that the technology can not only be used for seawater and freshwater nitrate isotope test, but also can be used for soil samples, and continue to improve and application of this technology was established in China, in the zonal and groundwater nitrate pollution research Made important progress. Due to many advantages of this technology, it is suggested to vigorously promote the research and application of this technology in our country. We hope relevant departments will step up their support to further improve this technology and promote its application.