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绝大部分灵长类动物存在与人类相似的ABO血型系统,该研究采用改良流式法(flow cytometry method,FCM)检测猕猴及食蟹猴血清中血型抗体水平的分布情况。以流式细胞术为基础,使用商品化人源红细胞为靶细胞,并通过加入特异性荧光标记的抗人IgM或IgG二抗,对收集的实验用猕猴及食蟹猴的血清样本进行检测,以人类健康受试者的血清样本为对照,比较两者血型抗体水平的差异。结果显示:预先用人O型浓缩红细胞吸附猴血清中所含种属间非特异性抗体后,FCM法能够准确检测其血型抗体水平及分型,并且发现猴血清中天然血型抗体的水平明显低于健康人(P<0.05)。由此得出:通过预处理清除非特异性抗体的干扰后,FCM法同样适用于灵长类动物血清中血型抗体的检测,也为构建灵长类动物模拟人ABO血型不合器官移植模型提供了技术保障和实验数据。
Most of the primates have human similar ABO blood group system. In this study, the flow cytometry (FCM) method was used to detect the distribution of blood group antibodies in macaque and cynomolgus monkey serum. On the basis of flow cytometry, commercial human erythrocytes were used as target cells, and serum samples from experimental cynomolgus monkeys and cynomolgus monkeys were detected by adding specific fluorescent-labeled anti-human IgM or IgG secondary antibody. Serum samples from healthy human subjects were used as controls to compare the differences of blood group antibody levels between the two groups. The results showed that FCM could accurately detect the level and type of blood group antibody after preadministration of non-specific interspecies antibodies in monkey serum with human O-type concentrated erythrocytes, and found that the level of natural blood group antibody in monkey serum was significantly lower than that of healthy Person (P <0.05). The result shows that the FCM method is also suitable for the detection of blood group antibodies in the primates serum by pretreatment to eliminate the interference of nonspecific antibodies, and also provides the technique for constructing primate analog human ABO incompatible organ transplantation models Safeguards and experimental data.