Three-dimensional Expansion： In Suspension Culture of SD Rat＇s Osteoblasts in a Rotating Wall Vesse

• 作 者: KE-DONG SONG TIAN-QINGLIU XIAN
• 机 构: StemCellandTissueEngineeringLaboratory,DepartmentofEngineeringScience
• 出 处: 生物医学与环境科学：英文版
• 发表日期: 2007年2期
• 关键词: SD大鼠 成骨细胞 悬浮培养 三维扩展 转壁式生物反应器 Osteoblast Bioreactor Microcarrier Tissue engine
• 基金项目: This work was supported by grants from the Int. Cooperation Project for National ＆ Abroad Lab. of the National Natural Sciences Foundation of China （No. 2002008） and The Science ＆ Technology Foundatio

Objective To study large-scale expansion of SD(Sprague-Dawley) rat's osteoblasts in suspension culture in a rotating wall vessel bioreactor(RWVB). Methods The bioreactor rotation speeds were adjusted in the range of 0 to 20 rpm,which could provide low