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目的:了解EB病毒相关感染患儿血EB病毒(EBV)DNA载量。方法:应用荧光定量PCR方法测定2009年6月～2011年6月在解放军空军总医院儿科住院的32例EB病毒相关感染患儿治疗前后外周血单个核细胞(PBMCs)EBV DNA载量。结果:EB病毒相关感染患儿血EBV DNA阳性率为100%,治疗后DNA载量明显减低。结论:PBMCs荧光定量PCR检测EBV DNA载量是EB病毒相关感染诊断和治疗的较好依据。
Objective: To understand the DNA load of Epstein-Barr virus (EBV) in children with Epstein-Barr virus infection. Methods: The EBV DNA load of peripheral blood mononuclear cells (PBMCs) from 32 children with Epstein-Barr virus infection who were hospitalized in PLA PLA PLA General Hospital from June 2009 to June 2011 was measured by real-time fluorescence quantitative PCR. Results: The positive rate of EBV DNA in children with Epstein-Barr virus-associated infection was 100%. The DNA load was significantly decreased after treatment. Conclusion: The detection of EBV DNA load by fluorescence quantitative PCR of PBMCs is a good basis for the diagnosis and treatment of Epstein-Barr virus-related infection.