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本实验利用铁氰化钾脉冲法和Ｒｈｏｄａｍｉｎｅ１２（Ｒｈ＿(１２３)）荧光染料流动透析法检测外源性黄嘌呤／黄嘌呤氧化酶系统产生对心肌线粒体跨膜电位及质子转位的作用，发现随－产生浓度的增加、能化后跨膜电位差逐渐减小，质子泵出速度降低及Ｈ￣＋／２ｅ￣－比值下降；随产生系统与线粒体共孵育时间的延长，能化后跨膜电位差逐渐减小，质子泵出速度降低，电子传递速度减小．Ｈ￣＋／２ｅ￣－比降低，即产生系统对Ｈ￣＋泵出及Ｈ￣＋／２ｅ￣－比的影响具有浓度依赖性和时间依赖性。丹参素对能化后膜电位差的下降有一定程度的恢复作用，推断对心肌线粒体膜的作用结果是使膜对质子的通透性增强，质子发生回漏，呼吸链电子传递酶系功能部分受损。
In this study, the effects of exogenous xanthine / xanthine oxidase system on mitochondrial transmembrane potential and proton translocation were examined by potassium ferricyanide pulse method and Rhodamine 12 (Rh 123) fluorescent dye flow dialysis method. - the increase of concentration, the transmembrane potential difference gradually decreases after protonation, the proton pumping speed decreases and the ratio of H ~ + / 2e ~ -decreases; with the generation system and mitochondria co-incubation time prolongation, transmembrane potential The difference decreases gradually, the proton pumping speed decreases and the electron transfer speed decreases. H ~ + / 2e ~ - ratio is lower, that is, the production system has a concentration-dependent and time-dependent effect on H ~ + pumping and H ~ + / 2e ~ Salvia miltiorrhiza on the reduction of membrane potential difference after a certain degree of recovery, inferred myocardial mitochondrial membrane effect is to make the membrane permeability of the proton enhanced proton leakage back, respiratory chain electron transport enzyme functional part Damaged.