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目的:探讨肝细胞生长因子(hepatocytegrowthfac-tor,HGF)在人晶状体上皮细胞(lensepitheliaclel,lLEC)的表达及其对晶状体上皮细胞的促增殖和抑制凋亡的作用。方法:取原代培养人晶状体上皮细胞,应用RT-PCR,Westernblot检测HGF,c-Met的mRNA及蛋白的表达,应用MTT法检测HGF对晶状体上皮细胞增殖的影响,Western-blo检t测Bcl-2的蛋白表达,以揭示其对晶状体上皮细胞凋亡的影响。结果:HGF和c-Met在人晶状体上皮细胞有表达,HGF有促进晶状体上皮细胞增殖的作用,并能抑制凋亡产生。结论:HGF参与人晶状体上皮细胞的增殖代谢过程,与晶状体上皮细胞的增殖相关。
AIM: To investigate the expression of hepatocyte growth factor (HGF) in human lens epithelial cells (lLEC) and its effect on the proliferation and apoptosis of lens epithelial cells. Methods: The primary culture of human lens epithelial cells was performed. The mRNA and protein expressions of HGF and c-Met were detected by RT-PCR and Western blot. The effect of HGF on the proliferation of lens epithelial cells was detected by MTT assay. The expression of Bcl-2 -2 protein expression in order to reveal its impact on lens epithelial cell apoptosis. Results: HGF and c-Met were expressed in human lens epithelial cells. HGF could promote the proliferation of lens epithelial cells and inhibit the apoptosis. Conclusion: HGF participates in the process of proliferation and metabolism of human lens epithelial cells, which is related to the proliferation of lens epithelial cells.