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为了解纤维连接蛋白(fibronectin ,Fn)在中耳粘连形成中的作用 ,采用新的火棉胶切片抗原恢复技术及超敏感的生物素 链霉卵白素法 (supersensitivebiotin streptavidin ,SSBSA)检测了粘连中耳在不同期Fn的含量。对结果采用光学显微镜下定性分析 :在中耳粘连各期Fn含量以阳性和强阳性为主 ,而正常组以阴性和弱阳性为主 ;计算机辅助定量分析 :正常组、纤维粘连期、细胞反应期、肉芽期、渗出期Fn含量吸光度 (A)值分别为(17 43±2 91)、(4 4 2 7± 4 5 0 )、(32 2 7± 8 33)、(2 8 91± 7 6 4)、(35 17± 14 2 8) ,正常组与粘连各期间有差异 (P <0 0 5 ) ,粘连各期间差异不明显 (P >0 0 5 )。提示Fn参与了中耳粘连的形成 ,其主要作用机制可能是促进粘连的始动物质———凝胶基质的形成。
In order to understand the role of fibronectin (Fn) in the formation of middle ear adhesions, we used a new pyroclastic patch antigen retrieval technique and ultrasensitive biosensin biotin streptavidin (SSBSA) Ear in different periods Fn content. Qualitative analysis of the results using optical microscopy: Fn content in the middle ear adhesions positive and strong positive, while the normal group of negative and weak positive; computer-assisted quantitative analysis: normal group, fibronectin, cell response The absorbency (A) values of Fn content in period, granulation period and exudation period were (17 43 ± 2 91), (442 7 ± 45 0), (32 27 ± 8 33), (28 91 ± (P <0.05). There was no significant difference between the normal group and the period of adhesion (P> 0.05). It is suggested that Fn is involved in the formation of middle ear adhesions. The main mechanism may be the formation of gel matrix, which is the initiating substance to promote adhesion.